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High levan accumulation in transgenic tobacco plants expressing the Gluconacetobacter diazotrophicus levansucrase gene
ArticleAbstract: Bacterial levansucrase (EC 2.4.1.10) converts sucrose into non-linear levan consisting of long β(2,6Palabras claves:FOS, Fructan, Gluconacetobacter diazotrophicus, Levan, Levansucrase, Transgenic tobaccoAutores:Arrieta J.G., Banguela A., Hernandez L., Luis E. Trujillo, Menéndez C., Rodríguez R.Fuentes:scopusFructo-oligosaccharides production by the Gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast Pichia pastoris
ArticleAbstract: Levansucrase (LsdA) (EC 2.4.1.10) from Gluconacetobacter diazotrophicus (formerly Acetobacter diazotPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Levansucrase, PICHIA PASTORIS, recombinant expressionAutores:Arrieta J.G., Dafhnis F., García J., Hernandez L., Luis E. Trujillo, Perez M., Támbara Y., Valdés J.Fuentes:scopusConstitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusCatalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
ArticleAbstract: The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltPalabras claves:Catalytical properties, Fructo-oligosaccharides, Fructosyltransferase, Levansucrase, N-glycosylation, PICHIA PASTORISAutores:Arrieta Sosa J.G., Banguela Castillo A., Gómez Riera R., Hernández García L., Luis E. Trujillo, Soto Romero M.Fuentes:scopusLevansucrase activity but not fructan accumulation in transgenic lsdA-expressing sugarcane recovered by optimized microprojectile bombardment of embryogenic calli
ArticleAbstract: Sugarcane (Saccharum spp. hybrid) emerges as an ideal crop for the cost-effective transgenic productPalabras claves:eGFP, Fructan, Genetic transformation, Levan, Levansucrase, LsdA, SugarcaneAutores:Arrieta J.G., Banguela A., Hernandez L., Kairúz E., Luis E. Trujillo, Menéndez C., Rodríguez R.Fuentes:scopusProduction of highly polymerized bacterial levan in two eukaryotic hosts of biotechnological interest
ArticleAbstract: Bacterial levan [β(2,6)-linked polyfructan] has potential applications in the food, bio-energetic, mPalabras claves:Catalytical properties, Fructan, Gluconacetobacter diazotrophicus, Levan, Levansucrase, N-glycosilation, PICHIA PASTORIS, Transgenic tobaccoAutores:Arrieta J.G., Banguela A., Borroto C., Enrique Pérez C.M., Hernandez L., Luis E. Trujillo, Pujol M., Ramírez R., Rodríguez R.Fuentes:scopus