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Complete sucrose hydrolysis by heat-killed recombinant Pichia pastoris cells entrapped in calcium alginate
ArticleAbstract: Background: An ideal immobilized biocatalyst for the industrial-scale production of invert sugar shoPalabras claves:Alginate, Immobilization, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Echemendia F.M., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Quintero Y., Ramírez R., Sobrino A.Fuentes:scopusDevelopment of soluble and immobilized Biocatalysts based on a recombinant thermostable β-fructosidase enabling complete sucrose inversion at pasteurization temperatures
ArticleAbstract: Biocatalysts for the industrial production of invert sugar are preferred to stably operate at high sPalabras claves:Immobilization, Invert syrup, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Basabe L., Cutiño-Ávila B.V., Del Monte-Martínez A., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Ramírez R., Sobrino A.Fuentes:scopusConstitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusConstitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris
ArticleAbstract: Enzymes for use in the sugar industry are preferred to be thermotolerant. In this study, a syntheticPalabras claves:Fructosidase, GAP promoter, Invert sugar, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:González E.M., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Pérez E.R.Fuentes:scopusCatalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
ArticleAbstract: The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltPalabras claves:Catalytical properties, Fructo-oligosaccharides, Fructosyltransferase, Levansucrase, N-glycosylation, PICHIA PASTORISAutores:Arrieta Sosa J.G., Banguela Castillo A., Gómez Riera R., Hernández García L., Luis E. Trujillo, Soto Romero M.Fuentes:scopusFructo-oligosaccharides production by the Gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast Pichia pastoris
ArticleAbstract: Levansucrase (LsdA) (EC 2.4.1.10) from Gluconacetobacter diazotrophicus (formerly Acetobacter diazotPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Levansucrase, PICHIA PASTORIS, recombinant expressionAutores:Arrieta J.G., Dafhnis F., García J., Hernandez L., Luis E. Trujillo, Perez M., Támbara Y., Valdés J.Fuentes:scopusFructooligosaccharides production by Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase constitutively expressed to high levels in Pichia pastoris
ArticleAbstract: The non-saccharolytic yeast Pichia pastoris was engineered to express constitutively the mature regiPalabras claves:1-kestose, FOS, Fructooligosaccharides, Fructosyltransferase, GAP promoter, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Musacchio Lasa A., Pérez E.R., Pimentel E., Ramírez R., Sobrino A.Fuentes:scopusFructooligosaccharides production by immobilized Pichia pastoris cells expressing Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase
ArticleAbstract: Fructooligosaccharides (FOSs)—fructose-based oligosaccharides—are typical prebiotics with health-proPalabras claves:Calcium alginate, Cell immobilization, Fructooligosaccharides, Fructosyltransferase, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Pimentel E., Ramírez R., Rodríguez I., Sobrino A.Fuentes:scopusOptimization of culture medium for large-scale production of heterologous proteins in Pichia pastoris to be used in nanoscience and other biotechnological fields
ArticleAbstract: The methylotrophic yeast Pichia pastoris became an excellent and successfully expression system forPalabras claves:Chemical defined medium, Heterologous protein expression system, PICHIA PASTORISAutores:José-Manuel Pais-Chanfrau, Luis E. TrujilloFuentes:googlescopusProduction of highly polymerized bacterial levan in two eukaryotic hosts of biotechnological interest
ArticleAbstract: Bacterial levan [β(2,6)-linked polyfructan] has potential applications in the food, bio-energetic, mPalabras claves:Catalytical properties, Fructan, Gluconacetobacter diazotrophicus, Levan, Levansucrase, N-glycosilation, PICHIA PASTORIS, Transgenic tobaccoAutores:Arrieta J.G., Banguela A., Borroto C., Enrique Pérez C.M., Hernandez L., Luis E. Trujillo, Pujol M., Ramírez R., Rodríguez R.Fuentes:scopus