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Biotecnologia Aplicada(3)
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Complete sucrose hydrolysis by heat-killed recombinant Pichia pastoris cells entrapped in calcium alginate
ArticleAbstract: Background: An ideal immobilized biocatalyst for the industrial-scale production of invert sugar shoPalabras claves:Alginate, Immobilization, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Echemendia F.M., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Quintero Y., Ramírez R., Sobrino A.Fuentes:scopusA pH shift-based procedure to screen fructoligosaccharides fermenting yeast or bacterial strains
ArticleAbstract: Symbiotic, a combination of short chains-carbohydrates with prebiotic properties e.g. fructooligosacPalabras claves:Bromothymol blue, Fructoligosaccharides, Fructoligosaccharides fermenting microorganisms, prebiotics, probiotics, screening, SymbioticAutores:Arrieta J.G., Brizuela M.A., Hernandez L., Luis E. Trujillo, Pérez E.R., Pérez H., Trujillo G.Fuentes:scopusDetection of deoxyribonuclease activities using a PCR-generated radiolabeled DNA substrate
ArticleAbstract: Here we evaluated a radioactive DNA substrate for the efficient detection of deoxyribonuclease activPalabras claves:Deoxyribonuclease activity, PCR, Quality controlAutores:Brito J., Dafhnis F., Luis E. Trujillo, Miranda F., Pérez E.R.Fuentes:scopusDevelopment of soluble and immobilized Biocatalysts based on a recombinant thermostable β-fructosidase enabling complete sucrose inversion at pasteurization temperatures
ArticleAbstract: Biocatalysts for the industrial production of invert sugar are preferred to stably operate at high sPalabras claves:Immobilization, Invert syrup, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Basabe L., Cutiño-Ávila B.V., Del Monte-Martínez A., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Ramírez R., Sobrino A.Fuentes:scopusConstitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris
ArticleAbstract: Enzymes for use in the sugar industry are preferred to be thermotolerant. In this study, a syntheticPalabras claves:Fructosidase, GAP promoter, Invert sugar, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:González E.M., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Pérez E.R.Fuentes:scopusCorrespondence between radioactive and functional methods in the quality control of DNA restriction and modifying enzymes
ArticleAbstract: We evaluated the use of two radiolabeled λ DNA/Hpa II substrates to detect 5'→3, 3'→5' single and doPalabras claves:Autores:González E.M., Luis E. Trujillo, Miranda F., Pérez E.R., Pupo E.Fuentes:scopusFructooligosaccharides production by Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase constitutively expressed to high levels in Pichia pastoris
ArticleAbstract: The non-saccharolytic yeast Pichia pastoris was engineered to express constitutively the mature regiPalabras claves:1-kestose, FOS, Fructooligosaccharides, Fructosyltransferase, GAP promoter, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Musacchio Lasa A., Pérez E.R., Pimentel E., Ramírez R., Sobrino A.Fuentes:scopusFructooligosaccharides production by immobilized Pichia pastoris cells expressing Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase
ArticleAbstract: Fructooligosaccharides (FOSs)—fructose-based oligosaccharides—are typical prebiotics with health-proPalabras claves:Calcium alginate, Cell immobilization, Fructooligosaccharides, Fructosyltransferase, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Pimentel E., Ramírez R., Rodríguez I., Sobrino A.Fuentes:scopusScaling-up batch conditions for efficient sucrose hydrolysis catalyzed by an immobilized recombinant Pichia pastoris cells in a stirrer tank reactor
ArticleAbstract: Background: Invert sugar is used greatly in food and pharmaceutical industries. This paper describesPalabras claves:Bioreactor, Calcium alginate beads, Enzymatic biocatalyst, Immobilized biocatalyst, Industrial biotechnology, Invert sugar, Invertase, Kinetic model, Scale-up, Stirred tank reactor, Sucrose inversionAutores:Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Rodríguez I., Sobrino A.Fuentes:scopus