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A pH shift-based procedure to screen fructoligosaccharides fermenting yeast or bacterial strains
ArticleAbstract: Symbiotic, a combination of short chains-carbohydrates with prebiotic properties e.g. fructooligosacPalabras claves:Bromothymol blue, Fructoligosaccharides, Fructoligosaccharides fermenting microorganisms, prebiotics, probiotics, screening, SymbioticAutores:Arrieta J.G., Brizuela M.A., Hernandez L., Luis E. Trujillo, Pérez E.R., Pérez H., Trujillo G.Fuentes:scopusA type II protein secretory pathway required for levansucrase secretion by Gluconacetobacter diazotrophicus
ArticleAbstract: The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levanPalabras claves:Autores:Alfonso D., Arrieta J.G., Hernandez L., Luis E. Trujillo, Menéndez C., Ramírez R., Soto M., Sotolongo M.Fuentes:scopusConstitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusFructo-oligosaccharides production by the Gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast Pichia pastoris
ArticleAbstract: Levansucrase (LsdA) (EC 2.4.1.10) from Gluconacetobacter diazotrophicus (formerly Acetobacter diazotPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Levansucrase, PICHIA PASTORIS, recombinant expressionAutores:Arrieta J.G., Dafhnis F., García J., Hernandez L., Luis E. Trujillo, Perez M., Támbara Y., Valdés J.Fuentes:scopusEngineering drought and salt tolerance in plants using SodERF3, a novel sugarcane ethylene responsive factor
ArticleAbstract: The ability of plants to tolerate salt and drought conditions is crucial for agricultural productionPalabras claves:Autores:Arrieta J.G., Banguela A., Borras-Hidalgo O., Coll Y., Hernández I., Hernandez L., Luis E. Trujillo, Menéndez C., Ochogavía M.E., Ramírez R., Rodríguez R.Fuentes:scopusHigh levan accumulation in transgenic tobacco plants expressing the Gluconacetobacter diazotrophicus levansucrase gene
ArticleAbstract: Bacterial levansucrase (EC 2.4.1.10) converts sucrose into non-linear levan consisting of long β(2,6Palabras claves:FOS, Fructan, Gluconacetobacter diazotrophicus, Levan, Levansucrase, Transgenic tobaccoAutores:Arrieta J.G., Banguela A., Hernandez L., Luis E. Trujillo, Menéndez C., Rodríguez R.Fuentes:scopusIdentification and recombinant expression of a bacterial exolevanase useful for the production of high fructose syrups
ArticleAbstract: The sugarcane endophyte Gluconacetobacter diazotrophicus produces levan from sucrose by a secreted lPalabras claves:Autores:Alfonso D., Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Menéndez C., Ramírez R.Fuentes:googlescopusLevansucrase activity but not fructan accumulation in transgenic lsdA-expressing sugarcane recovered by optimized microprojectile bombardment of embryogenic calli
ArticleAbstract: Sugarcane (Saccharum spp. hybrid) emerges as an ideal crop for the cost-effective transgenic productPalabras claves:eGFP, Fructan, Genetic transformation, Levan, Levansucrase, LsdA, SugarcaneAutores:Arrieta J.G., Banguela A., Hernandez L., Kairúz E., Luis E. Trujillo, Menéndez C., Rodríguez R.Fuentes:scopusRecombinant expression of active Penicillium minioluteum endodextranase in alternative hosts
ArticleAbstract: The dextranase gene from the fungus Penicillium minioluteum was expressed in Escherichia coli. The rPalabras claves:Cell bombardment, Dextranase, ESCHERICHIA coli, Penicillium minioluteum, recombinant expressionAutores:Arrieta J.G., Dafhnis F., Fuentes A.D., Garcia B.M., Hernandez L., Luis E. Trujillo, Quintero O., Ramos P.L.Fuentes:scopusProduction of highly polymerized bacterial levan in two eukaryotic hosts of biotechnological interest
ArticleAbstract: Bacterial levan [β(2,6)-linked polyfructan] has potential applications in the food, bio-energetic, mPalabras claves:Catalytical properties, Fructan, Gluconacetobacter diazotrophicus, Levan, Levansucrase, N-glycosilation, PICHIA PASTORIS, Transgenic tobaccoAutores:Arrieta J.G., Banguela A., Borroto C., Enrique Pérez C.M., Hernandez L., Luis E. Trujillo, Pujol M., Ramírez R., Rodríguez R.Fuentes:scopus