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"Reverse-staining" of biomolecules in electrophoresis gels: Analytical and micropreparative applications
ReviewAbstract: Negative or reverse staining using imidazole and zinc salts for protein detection in electrophoresisPalabras claves:Gel electrophoresis, lipopolysaccharides, Negative staining of gels, Nucleic acids, proteins, Proteomic purification, Proteomic sample preparation, Reverse staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusA procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusFast passive elution of DNA from zinc-imidazole negatively stained polyacrylamide gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Sánchez J.C.Fuentes:scopusNegative detection of biomolecules separated in polyacrylamide electrophoresis gels
ArticleAbstract: Here we describe the protocols for negative or reverse detection of proteins, nucleic acids and lipoPalabras claves:Autores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusImproving the expression of mini-proinsulin in Pichia pastoris
ArticleAbstract: Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achiPalabras claves:AOX promoter, fed-batch culture, PICHIA PASTORIS, Recombinant human insulinAutores:Besada-Pérez V., Cabello C., Castellanos‐Serra L., Del C. Abrahantes-Pérez M., Del Toro Y., García Y., Hernández L., Hidalgo A., José-Manuel Pais-Chanfrau, Licor L., Mansur M., Martinez E., Plana L., Támbara Y., Valdés J.Fuentes:googlescopusMice immunization with gel electrophoresis-micropurified bacterial lipopolysaccharides
ArticleAbstract: Some evidence on the possible use of sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDSPalabras claves:Elution, Gel electrophoresis, Immunization, lipopolysaccharides, Zinc stainingAutores:Aguila A., Castellanos‐Serra L., Eugenio Hardy, Núñez J.F., Pupo E., Santana-Milián H.Fuentes:scopusSensitive reverse staining of bacterial lipopolysaccharides on polyacrylamide gels by using zinc and imidazole salts
ArticleAbstract: We present a new method for visualizing bacterial lipopolysaccharides (LPS)/lipooligosaccharides (LOPalabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Pupo E., Reyes J.Fuentes:scopus