Proteomics of multigenic families from species under represented in databases: The case of loquat (eriobotrya japonica lindl.) polyphenol oxidases

Abstract:

Here, we approach the problem of obtaining accurate and reliable information about the gene origin of a protein belonging to a multigenic family, polyphenol oxidase, from an underrepresented species, Eriobotrya japonica. De novo sequencing was a key approach to obtain broad sequence coverage. Alignment of peptides on their most similar homologous protein revealed divergent amino acid positions that lead to hypothesize the minimal number of genes encoding for the proteins analyzed. © 2008 American Chemical Society.

Año de publicación:

2008

Keywords:

• polyphenol oxidase
• Phylogenetic analysis
• Underrepresented species in databases
• Multigenic families
• de novo sequencing

Fuente:

scopus