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Rapid quantification of riboflavin in milk by front-face fluorescence spectroscopy: A preliminary study

Abstract:

The front-face fluorescence spectroscopy technique was used to establish a rapid pbkp_rediction model of riboflavin concentration in milk without prior sample preparation. The pbkp_rediction model developed was then compared with two conventional high performance liquid chromatography (HPLC)-based quantification methods. The method of standard addition allowed detecting a linear correlation between fluorescence intensity and riboflavin concentration in 12% (w/w) reconstituted low-heat milk powder. Validation of the model yielded an R2 of 0.99 with a standard error of pbkp_rediction of 0.13 mg/L. The results suggest a potential use of front-face fluorescence spectroscopy as a simple method for off- and in-line determinations of riboflavin in milk.