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"Reverse-staining" of biomolecules in electrophoresis gels: Analytical and micropreparative applications
ReviewAbstract: Negative or reverse staining using imidazole and zinc salts for protein detection in electrophoresisPalabras claves:Gel electrophoresis, lipopolysaccharides, Negative staining of gels, Nucleic acids, proteins, Proteomic purification, Proteomic sample preparation, Reverse staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusA New Science Publishing System for a Budding Science Publishing Crisis
ArticleAbstract: The current science publishing system is in need of a positive transformation for the good of scientPalabras claves:Journal, METRICS, Open access, Science publishingAutores:Eugenio Hardy, Fernandez‐Patron C.Fuentes:scopusA sandwich-type enzyme-linked immunosorbent assay for the analysis of recombinant human interferon α-2b
ArticleAbstract: The ability of a previously developed sandwich-type enzyme-linked immunosorbent assay (ELISA) for diPalabras claves:Elisa, ESCHERICHIA coli, folding, IFN-alpha, monoclonal antibodiesAutores:Espino Y., Eugenio Hardy, Franco A., Furrazola G., Santana-Milián H.Fuentes:scopusA synthetic conjugate polysaccharide vaccine against haemophilus influenzae type b
ArticleAbstract: Glycoconjugate vaccines provide effective prophylaxis against bacterial infections. To date, howeverPalabras claves:Autores:Aguilar A., Baly A., Campa C., Cardoso F., Carmenates A., Cosme K., Costa L., Deler M.L., Diaz M., Eugenio Hardy, Fernández-Santana V., Garcia E., Hernández I., Herrera L., Heynngnezz L., Izquierdo M., Medina E., Montane M., Muzachio A., Ramos A., Rodriguez A., Rodriguez M.C., Roy R., Sánchez R., Sosa I., Toledo-Romani M.E., Toraño G., Valdés Y., Verez-Bencomo V., Villar A.Fuentes:scopusComparison of two gel electrophoresis-based DNA micropurification methods for molecular cloning
ArticleAbstract: We recently described a method for recovering nucleic acids separated from polyacrylamide gels, whicPalabras claves:Cloning, Ethidium bromide, Gel electrophoresis, Ultraviolet-light, Zinc stainingAutores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Hermida L., Niebla O., Pupo E., Silva R.Fuentes:scopusComplexity and solutions to the isolation problem of Gram negative lipopolysaccharides' bacteria molecular species
ReviewAbstract: Gram negative bacteria frequently synthesize a great number of lipopolysaccharides (LPS) molecular sPalabras claves:chromatography, Electrophoresis, Elution, Isolation, lipopolysaccharides, Mass spectrometry, Reverse stainingAutores:Eugenio Hardy, Pupo E.Fuentes:scopusBiopharmaceutical formulations of liposome-associated desoxyribonucleic acids
ReviewAbstract: In this paper the current development of liposome-based DNA formulations as a biopharmaceutical prodPalabras claves:delivery system, Gene therapy, LiposomeAutores:Eugenio Hardy, Pupo E.Fuentes:scopusCould Endogenous Glucocorticoids Influence SARS-CoV-2 Infectivity?
ArticleAbstract: Endogenous glucocorticoids and their synthetic analogues, such as dexamethasone, stimulate receptor-Palabras claves:ACE2 (angiotensin converting enzyme 2), cortisol, covid-19, Dexamethasone, glucocorticoids, innate immunity, SARS-COV-2, Spike proteinAutores:Eugenio Hardy, Fernandez‐Patron C.Fuentes:scopusActive immunization with human interleukin-15 induces neutralizing antibodies in non-human primates
ArticleAbstract: Background: Interleukin-15 is an immunostimulatory cytokine overexpressed in several autoimmune andPalabras claves:Alum, CTLL-2 cells, Cytokine, IL-15, Immunization, Neutralizing Abs, non-human primatesAutores:Besada-Pérez V., Castro-Velazco J., Chico-Capote A., Eugenio Hardy, Gerónimo-Pérez H., Martínez-Castillo R., Martínez-Cordovez K., Morera-Díaz Y., Puente-Pérez P., Rodríguez-Álvarez Y., Santos-Savio A.Fuentes:scopusDouble Staining of Coomassie Blue-Stained Polyacrylamide Gels by Imidazole-Sodium Dodecyl Sulfate-Zinc Reverse Staining: Sensitive Detection of Coomassie Blue-Undetected Proteins
ArticleAbstract: The sensitivity, simplicity, and relative rapidity of Coomassie blue staining have made this techniqPalabras claves:Autores:Castellanos L.R., Eugenio Hardy, Fernandez‐Patron C., Seoane J., Sosa A.E.Fuentes:scopus