Mostrando 10 resultados de: 12
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Complete sucrose hydrolysis by heat-killed recombinant Pichia pastoris cells entrapped in calcium alginate
ArticleAbstract: Background: An ideal immobilized biocatalyst for the industrial-scale production of invert sugar shoPalabras claves:Alginate, Immobilization, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Echemendia F.M., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Quintero Y., Ramírez R., Sobrino A.Fuentes:scopusA protocol for the purification of bovine serum albumin free of deoxyribonuclease activity
ArticleAbstract: Bovine serum albumin, free of deoxyribonuclease activity, was obtained in our laboratory using ion-ePalabras claves:Autores:Castellanos L.R., Garcia O., Herrera L., Luis E. TrujilloFuentes:scopusDetection of deoxyribonuclease activities using a PCR-generated radiolabeled DNA substrate
ArticleAbstract: Here we evaluated a radioactive DNA substrate for the efficient detection of deoxyribonuclease activPalabras claves:Deoxyribonuclease activity, PCR, Quality controlAutores:Brito J., Dafhnis F., Luis E. Trujillo, Miranda F., Pérez E.R.Fuentes:scopusDevelopment of soluble and immobilized Biocatalysts based on a recombinant thermostable β-fructosidase enabling complete sucrose inversion at pasteurization temperatures
ArticleAbstract: Biocatalysts for the industrial production of invert sugar are preferred to stably operate at high sPalabras claves:Immobilization, Invert syrup, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Basabe L., Cutiño-Ávila B.V., Del Monte-Martínez A., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Ramírez R., Sobrino A.Fuentes:scopusConstitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusCorrespondence between radioactive and functional methods in the quality control of DNA restriction and modifying enzymes
ArticleAbstract: We evaluated the use of two radiolabeled λ DNA/Hpa II substrates to detect 5'→3, 3'→5' single and doPalabras claves:Autores:González E.M., Luis E. Trujillo, Miranda F., Pérez E.R., Pupo E.Fuentes:scopusCatalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
ArticleAbstract: The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltPalabras claves:Catalytical properties, Fructo-oligosaccharides, Fructosyltransferase, Levansucrase, N-glycosylation, PICHIA PASTORISAutores:Arrieta Sosa J.G., Banguela Castillo A., Gómez Riera R., Hernández García L., Luis E. Trujillo, Soto Romero M.Fuentes:scopusFrom the laboratory to the industry: Enzymatic production and applications of shortchain fructooligosaccharides (FOS). Recent advances and current perspectives
ReviewAbstract: The production of high yields of oligosaccharides of specific chain length from simple raw materialsPalabras claves:Enzymatic production, Enzymes, oligosaccharidesAutores:L. K. Ponce, Luis E. Trujillo, Rodrigo Ávalos, Thelvia I. Ramos Gómez, Vanessa E. MarcilloFuentes:scopusFructo-oligosaccharides production by the Gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast Pichia pastoris
ArticleAbstract: Levansucrase (LsdA) (EC 2.4.1.10) from Gluconacetobacter diazotrophicus (formerly Acetobacter diazotPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Levansucrase, PICHIA PASTORIS, recombinant expressionAutores:Arrieta J.G., Dafhnis F., García J., Hernandez L., Luis E. Trujillo, Perez M., Támbara Y., Valdés J.Fuentes:scopusFructosyltransferases and invertases: Useful enzymes in the food and feed industries
Book PartAbstract: Enzymes are very popular and well-established biotechnology products with a great and growing globalPalabras claves:Bioprocess, Cell immobilization, Enzymes, FOS, GOS, Inverted sugarAutores:Cruz E.P., García D.M., Jimmy Núñez Pérez, José-Manuel Pais-Chanfrau, Leonor Margarita Rivera Intriago, Luis E. TrujilloFuentes:scopus