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A protocol for the purification of bovine serum albumin free of deoxyribonuclease activity
ArticleAbstract: Bovine serum albumin, free of deoxyribonuclease activity, was obtained in our laboratory using ion-ePalabras claves:Autores:Castellanos L.R., Garcia O., Herrera L., Luis E. TrujilloFuentes:scopusA type II protein secretory pathway required for levansucrase secretion by Gluconacetobacter diazotrophicus
ArticleAbstract: The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levanPalabras claves:Autores:Alfonso D., Arrieta J.G., Hernandez L., Luis E. Trujillo, Menéndez C., Ramírez R., Soto M., Sotolongo M.Fuentes:scopusDetection of deoxyribonuclease activities using a PCR-generated radiolabeled DNA substrate
ArticleAbstract: Here we evaluated a radioactive DNA substrate for the efficient detection of deoxyribonuclease activPalabras claves:Deoxyribonuclease activity, PCR, Quality controlAutores:Brito J., Dafhnis F., Luis E. Trujillo, Miranda F., Pérez E.R.Fuentes:scopusDevelopment of soluble and immobilized Biocatalysts based on a recombinant thermostable β-fructosidase enabling complete sucrose inversion at pasteurization temperatures
ArticleAbstract: Biocatalysts for the industrial production of invert sugar are preferred to stably operate at high sPalabras claves:Immobilization, Invert syrup, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Basabe L., Cutiño-Ávila B.V., Del Monte-Martínez A., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Ramírez R., Sobrino A.Fuentes:scopusConstitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris
ArticleAbstract: Enzymes for use in the sugar industry are preferred to be thermotolerant. In this study, a syntheticPalabras claves:Fructosidase, GAP promoter, Invert sugar, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:González E.M., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Pérez E.R.Fuentes:scopusCorrespondence between radioactive and functional methods in the quality control of DNA restriction and modifying enzymes
ArticleAbstract: We evaluated the use of two radiolabeled λ DNA/Hpa II substrates to detect 5'→3, 3'→5' single and doPalabras claves:Autores:González E.M., Luis E. Trujillo, Miranda F., Pérez E.R., Pupo E.Fuentes:scopusCatalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
ArticleAbstract: The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltPalabras claves:Catalytical properties, Fructo-oligosaccharides, Fructosyltransferase, Levansucrase, N-glycosylation, PICHIA PASTORISAutores:Arrieta Sosa J.G., Banguela Castillo A., Gómez Riera R., Hernández García L., Luis E. Trujillo, Soto Romero M.Fuentes:scopusFructooligosaccharides production by Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase constitutively expressed to high levels in Pichia pastoris
ArticleAbstract: The non-saccharolytic yeast Pichia pastoris was engineered to express constitutively the mature regiPalabras claves:1-kestose, FOS, Fructooligosaccharides, Fructosyltransferase, GAP promoter, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Musacchio Lasa A., Pérez E.R., Pimentel E., Ramírez R., Sobrino A.Fuentes:scopusNegative staining with zinc-imidazole of gel electrophoresis-separated nucleic acids
ArticleAbstract: Zinc and imidazole salts were applied for the detection of nucleic acids on either polyacrylamide orPalabras claves:Agarose gel electrophoresis, Detection of nucleic acids, Negative staining, Zinc staining, Zinc-imidazole detectionAutores:Casalvilla R., Castellanos‐Serra L., Eugenio Hardy, López E., Luis E. Trujillo, Pupo E., Sosa A.E.Fuentes:scopusNmDef02, a novel antimicrobial gene isolated from Nicotiana megalosiphon confers high-level pathogen resistance under greenhouse and field conditions
ArticleAbstract: Plant defensins are small cysteine-rich peptides that inhibit the growth of a broad range of microbePalabras claves:Disease control, Plant defensin, Recombinant proteins, Transgenic plantsAutores:Ayra C., Borras-Hidalgo O., Borroto C., Castillo J., Chacón O., Enriquez G.A., Gallo A., González E.M., López Y., Luis E. Trujillo, Portieles R., Pujol M., Rodriguez M., Rodríguez R., Thomma B.P.H.J.Fuentes:scopus