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Isolation of baby lima bean (Phaseolus lunatus) proteins fractions and evaluation of their antioxidant activity

Abstract:

The aim of this work was to obtain fractions of baby Lima Bean Protein Concentrate (LBPC) from (Phasedus lunatus) and to evaluate their antioxidant activity. LBPC was prepared bv alkaline extraction and isoelectric precipitation at pH 4.3. LBPC was subject to gastrointestinal digestion simulation. LBPC 'was fractionated using a DEAE Affi-Gel Blue Gel column. LBPC presented a protein content of 77.20% with a protein solubility capacity ranging between 37.34% to 99.98%. The antioxidant activity was evaluated using the TRAP, DPPH and ORAC methods. LBPC cationic fractions presented a high antioxidant activity when using the ORAC method with values ranging between 0.47 to 3.37 μmol TE/μmol of sample.