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Evolution of Trypsin Inhibitor Activity during Germination of Lentils

Abstract:

The effect of light and seed rinsing during the germination of lentil seeds (Lens culinarie var. Vulgaris cv. Magda-20) on trypsin inhibitor activity (TIA) has been studied. TLA decreased progressively during germination, while hydration grade and light exposure had less influence on TIA. In general, there was no significant change in TIA after 3 days of germination, except when seeds were rinsed only once and the experiment was conducted in the dark, showing a 10% TIA decrease. Six-day germination produced TIA decreases from 7% to 18%. In this case, the number of rinses had no significant effect on the final TIA, but the activity of protease inhibitors was significantly higher in seeds illuminated for 6 h per day. Maximal TIA decreases were found in seeds germinated in darkness and rinsed daily. Under these optimum conditions, TIA first decreased slowly (12% loss of this activity after 6 days) followed by a faster decrease (up to 45% of control seeds values) after 10 days of germination. © 1995, American Chemical Society. All rights reserved.